Day 2 :
Keynote Forum
K Pani Prasad
ICAR-Central Institute of Fisheries Education, India
Keynote: Edible antibodies (IgY) in health management
Biography:
K. Pani Prasad is result oriented with 24 years of experience as a Professor, researcher, scientist, extension and administration worker in areas of aquatic animal health especially in areas of development and use of disease diagnostics and control. More than 100 research publications in peer-reviewed national and international journals with high impact factor. Presently working on projects as Principal Investigators: Aquatic disease surveillance project funded by NFDB, GOI; Networking project on Fish Health funded by ICAR, New Delhi; Development of nanotechnology-based diagnostic kit for detection of Betanoda virus in fish funded by CIFE, Mumbai; Network project on AMR with special reference to aquaculture and fisheries.
Abstract:
Although, aquaculture has developed significantly, management of diseases needs more emphasis. Specific antibodies for designing and use of different immunodiagnostics are necessary. Secondly, there is a great scope for integration of passive immunization i.e. IgY immunotherapy, the concept in health management needs to be tapped. Specific antibodies produced in chickens offer several important advantages over producing antibodies in other mammals. A single egg contains as much antibody as an average 20 ml bleed from a rabbit. This simple, non-invasive approach presents an appealing alternative to conventional polyclonal antibodies production methods. In addition, the eggs from immunized chickens provide a continual, daily source of antibody and this convenient approach offers greater compatibility with animal protection regulations. These IgY antibodies could be used for either developing immunodiagnostic kits or in passive immunotherapy against diseases. The IgY antibodies are stable in the intestinal tract of humans and animals and many reports are available. These antibodies are used in the prevention of many bacterial and viral diseases viz. gingivitis, dental caries, gastritis, gastric ulcers, infant rotavirus diarrhea in humans; livestock diseases such as mastitis and diarrhea, poultry diseases such as Salmonella, Campylobacteriosis, infectious bursal disease and Newcastle disease; as well as aquatic diseases like shrimp white spot syndrome virus, Yersinia ruckeri and Edwardsiella tarda. The positive results have led to penetrate the market of novel nutraceuticals and health supplements. This will reduce the use of chemicals/antibiotics, etc. in the environment and also help in controlling infections. With the use of these natural products of IgY, there would be increased use of these functional foods in the treatment and control of diseases at a reduced cost with no side effects to the body.
- : Food Processing | Food Chemistry | Food Microbiology | Food Technology
Location: Radisson Blu Hotel, Abu Dhabi Yas Island | Abu Dhabi, UAE
Chair
K Pani Prasad
ICAR-Central Institute of Fisheries Education, India
Session Introduction
Preeti Ahluwalia
Punjab Agricultural University, India
Title: Effect of packaging materials on quality of Quinoa enriched cereal bars
Biography:
Preeti Ahluwalia working as associate professor in the department of food science and technology, Punjab Agricultural University, Ludhiana. I was born and brought up in Ludhiana, Punjab, India. I did my schooling from a local convent school. After school, I did my bachelor’s degree in home science and and my master’s and doctorate in food science and technology. As a teacher I have taught many courses in the field of food technology and to name them a few; processing of cereals, pulses and oilseeds, processing of fruits and vegetables, sensory evaluation of foods, food packaging. My area of interest of work is developing foods for diabetics and celiac patients as there has been a tremendous rise in population suffering from these two diseases over a period of last decade. I have developed gluten free pasta from little millet and an energy bar with quinoa as a major ingredient for celiac patients. Flour with low GI for making chapati was developed which has been commercialised. I plan to continue working on these type of products.
Abstract:
Quinoa based bar was developed using quinoa, brown rice, flaxseed and nuts and honey (50%) was added as a sweetener and binding agent. The shelf life of bar was assessed in Aluminum Laminates (AL) and High Density Polyethylene (HDPE) bags stored under ambient temperature conditions for 4 months. The stored bar samples were analyzed for moisture content, peroxide value, free fatty acid and sensory quality. Significant variation was noticed in the free fatty acid and peroxide value of cereal bar stored in HDPE packaging material as compared to aluminum laminate bags. However the increase in Peroxide Value (PV; 1.69%) and Free Fatty Acid (FFA; 0.96%) was lesser even in HDPE bags than the prescribed limit. The data related to PV, FFA, moisture content and organoleptic quality of bar using different packaging materials during storage revealed that, the bar remained stable and acceptable for 4 months at ambient conditions irrespective of the packaging materials used. However AL was found to be better packaging material as compared to HDPE because of the better retention of chemical and sensory quality. Product was acceptable after the storage period of 120 days.
Reshu Rajput
Punjab Agricultural University, India
Title: Variability in the functional properties, amino acid composition and molecular weight distribution of the soy protein isolates obtained from six different cultivars of Punjab and Madhya Pradesh
Biography:
Reshu Rajput is currently pursuing PhD in the Department of Food Science and Technology at Punjab Agricultural University, Ludhiana under the guidance of Dr. Amarjeet Kaur. She has completed her Master’s degree from Pondicherry University, India. She has published a research article in Journal of Food Science and Technology in the year 2015.
Abstract:
The soy protein isolates were prepared by isoelectric precipitation and evaluated for various functional properties such as water absorption capacity, oil absorption capacity, water solubility index, swelling power, foaming capacity, foaming stability, bulk density and least gelation concentration of different soybean cultivars SL 744, SL 958, SL 525, JS 335, JS 93-05, JS 97-52 grown in Punjab and Madhya Pradesh. The oil absorption capacity ranged from 1.86 to 2.18 g/g and highest was found to be in JS 93-05 i.e. 2.18 g/g. The water absorption capacity ranged from 0.993 to 3.67 g/g and found to be higher in JS 335 i.e. 3.67 g/g. the emulsion capacity varied from 5 to 24% and JS 335 showed the highest emulsion capacity (24%). The least gel concentration of protein isolates ranged between 12 to 16%. Foam capacity significantly varied from 3.33 to 29.80% and highest was observed in SL 958. The amino acid composition was analyzed by GC-MS and the results showed that among essential amino acid phenylalanine and histidine (14 to 16%) has the highest concentration and among non-essential amino acid tyrosine has the highest concentration (around 30%). The molecular weight determination was done through SDS-PAGE. The major portion of total soy proteins consists of glycinin (11S) and β-conglycinin (7S) proteins and the molecular weight range between 37 kDa to 42 kDa for 11S and 42 kDa to 57 kDa for 7S. The functional properties evaluation revealed that Madhya Pradesh varieties possess good functional properties as compared to Punjab varieties and among Punjab varieties SL 958 was found to be at par with Madhya Pradesh soybean varieties.
Akashdeep Kaur
Punjab Agricultural University, India
Title: Development of flat bread fortified with fresh, paste and dried (powder) form of red bell pepper
Biography:
Akashdeep Kaur has completed Bachelor of Science in Home Science in 2017 from Punjab Agricultural University, Ludhiana. Currently she is pursuing Master of Science in Food Technology in Punjab Agricultural University.
Abstract:
In this study, the effect of incorporation of various forms of red bell pepper (fresh, powder and paste) on the sensory characteristics, consumer acceptability, texture, color and nutritional quality of flat bread was evaluated. Fresh bell pepper, dried bell pepper and paste were used in bread at different substitution levels: Dried at the levels of 1.5%, 2% and 5%; paste at the levels of 10%, 15% and 20% and fresh bell pepper at the levels of 10%, 15% and 20% (wt/wt of wheat flour). The dried powder at the level of 1.5%, paste at the level of 20% and fresh bell pepper at the level of 20% were selected for product formation as they provided good texture, good flavor, better product quality and high nutritional value. The product was analyzed for various quality characteristics viz. total phenols, antioxidants, protein, fat, ash, moisture and texture, crude fiber, carotenoids, antimicrobial activity, yeast and mold counts. The product was found to have high functional value due to the high content of phytochemicals like carotenoid and total phenols. The bread enriched with fresh red bell pepper (23.71% M.C, 0.004% A.C, 5.41% F.C), dry red bell pepper powder (21.81% M.C, 0.006% A.C, 10.48% F.C) and red bell pepper paste (21.81% M.C, 0.005% A.C, 5.91% F.C) has high moisture, ash and fiber content as compared to control bread (19.05% M.C, 0.001% A.C, 3.45% F.C). Product prepared with powder (1.5%) had high phenolic content and antioxidant activity as compared to other breads which incorporated with fresh red bell pepper (20%) and paste (20%). Product (bread) incorporated with paste of red bell pepper has good appearance and bright in color. Fresh bell pepper product had high sensory scores as compared to others. According to these results, the use of bell pepper can enhance nutritional quality as well as the sensory characteristics of bread.
Ramandeep Kaur
Punjab Agricultural University, India
Title: Quality evaluation of bread enriched with red sweet pepper powder (Capsicum annuum)
Biography:
Ramandeep Kaur has completed BS (Bachelor of Science) in food science and technology from Guru Nanak Dev University and MS (Master of Science) in Food technology from Punjab Agricultural University, India. Currently she is doing Ph.D in Food Technology in Punjab Agricultural University, India. She has attended 3 international and 3 national conferences.
Abstract:
Bread is an ideal vehicle to impart bioactive compounds to the consumers in a convenient manner. This study evaluated bread enriched with Red Sweet Pepper powder (RSP) at 2, 4, 6, 8 and 10% compared to control bread (without RSP). The bread crumbs were assayed for bioactive, physical, nutritional, textural, color and sensory properties. Bread supplemented with RSP improved its color, nutritional and bioactive properties. The low moisture content and increased hardness were observed at higher levels of RSP. Color intensity (expressed as L*, a*, b* values) of bread with 2 and 4% RSP were lower than those of high levels and the same trend was observed for protein, fiber and ash content of bread. Significant (p<0.05) increases were recorded for bioactive compounds such as total phenols (0.145 to 235 mg GAE/g), antioxidant activity (56% to 78%) and flavonoids (0.112 to 0.379 mg/g) as the level of powder increased. Bread enriched with 8% RSP showed improved sensory profile as compared to control, whereas a further increase in RSP decreased the sensory and textural properties. Thus, RSP act as a natural colorant and functional food that enhanced the functional and nutritional properties of bread and can be used to customize bread for specific health needs.
Shameema Vattamkandathil
Madras University, India
Title: Impact of food and nutrition in life style modification
Biography:
Shameema Vattamkandathil is a Centre Manager and Clinical Nutritionist. She has completed her Graduation in Food Science and Quality Control from Mahatma Gandhi University, India and Post-graduation in Food Chemistry and Food Processing from Loyola College, Chennai, India with an additional Master of Science in Applied Psychology and Master of Business Administration from Bharathiar University, Coimbatore, India.
Abstract:
Food and nutrition are the prime source of energy for our body. Food intakes play an important role in body weight. Nutrition is defined as the process in which animal or plant takes in and utilizes to release energy from the body. It is essential for growth and development, health and wellbeing. These are assessed by bodyweight which categorize by body composition analysis. Relationship between food nutrition and body weight includes a discussion of how the body weight can be maintained with a healthy balanced eating habit and lifestyle modification. The factors affecting the eating habits depend on the time, quality and quantity of food. The urge to eat is controlled by gut hormones and brain, mainly hunger hormones and satiety hormones, after a sustained weight loss within two years, the satiety hormones are override by hunger hormones, by a feeling of hunger which tends to have more food is influenced by factors such as food's savory appeal, the body's fat stores and need of energy. For decades, scientists have been researching the role of two hormones called leptin and ghrelin in regulating hunger, food preferences and satiety as signals from the body to the brain and seem to regulate appetite and satiety. Life style correction to manage body weight are dietary plans, physical activity and behavioral therapy, which helps the complete transformation of the individual. Attitude towards the eating, exercise and behavior therapy are essential for a sustained weight reduction. Life style changes leads to obesity, which is a chronic medical condition. Obesity in adulthood leads to diabetes, high blood pressure, cardio vascular disease, gallstones, strokes, risk factor for a number of cancers which is associated with a striking reduction in life expectancy for both men and women so that a healthy lifestyle correction is required. For a healthy life style modification wellness centers are recommended to give guidelines and motivational support to the individual in order to change their attitude, awareness and authenticity towards a healthy living.
Dolly
Punjab Agricultural University, India
Title: Effect of physicochemical, thermal and rheological properties on the extrusion behavior of the eminent Indian rice cultivars
Biography:
Dolly is currently pursuing her PhD in Food Science and Technology as a Junior Research Fellow from Punjab Agriculture University, Punjab. She has completed her Masters from Indian Council of Agriculture Research.
Abstract:
The objective of present investigation was to assess the effect of physicochemical, thermal and rheological properties of the eminent Indian rice cultivars (PR-114, PR-121, PR-122, PR-123, PR-124, PR-126 and PR- 127) on their extrusion behavior. Rheological properties such as pasting temperature, peak, hold, breakdown, setback and final viscosities were evaluated using Rapid Visco Analyzer that in turn main set a significant impact over the extrusion behavior of the selected cultivars. Cultivars like PR-122 and PR-124 categorically demonstrated a high water solubility index of 48.52 and 41.86 percent due to the presence of high amylose content i.e., 28.86 and 26.14 percent respectively. A noteworthy variation was observed in the expansion ratio of the resultant extrudates which is correlated with the degree of gelatinization because it is directly dependent on the granule structure destruction. It is one of the most important product responses that play a crucial role in governing the acceptability of the product. Gelatinization temperature showed significant positive correlation with amylose content and pasting temperature. A significant variation was testified in the bulk density of the resultant extrudates. These broadly diversified and anticipated physicochemical, thermal and rheological properties of the Indian cultivars possess significant impact on their extrusion behavior. Their detailed information can establish the foundation for obtaining extruded products with more acceptable and anticipated characteristics.
Remya Radha
Indian Institute of Technology Madras, India
Title: Thermal stability studies of Vibrio cholerae L-asparaginase: Deactivation kinetics and stability enhancement
Biography:
Abstract:
Studies on deactivation kinetics as well as quantification of temperature dependent macromolecular properties of protein like activity and stability are ineluctable for predicting economic feasibility for industrial applications. L-asparaginase (L-asparagine amidohydrolase, E.C. 3.5.1.1) is an industrially important enzyme which catalyzes the conversion of L-asparagine to L-aspartate and ammonia. This enzyme has been using as a food processing enzyme as it can effectively reduce the level of acrylamide in a range of starchy foods. L-asparaginase also has application in the treatment of acute lymphoblastic leukemia and its clinical action is attributed to the depletion of L-asparagine, since tumor cells are impotent to synthesize this amino acid and are selectively killed by L-asparagine deprivation. This study reports the temperature dependent deactivation kinetics and stability studies of Vibrio cholerae L-asparaginase. Circular dichroism and differential scanning calorimetry studies have been carried out to understand the temperature-dependent conformational changes in the secondary structure of V. cholerae L-asparaginase. Thermal stability studies on V. cholerae L-asparaginase revealed that the protein is stable up to 40 °C, retaining 70-85% residual activity even after 6 hours of incubation. Moreover it possessed very high melting point of 81 °C and high half-life time of 1100 minutes at 37 °C. Protein was found to be less stable at higher temperatures of 45, 50 and 55 °C and residual activity was declined to 1-6 % in 15 minutes. The effect of various thermal additives like glycerol, glycine and trehalose on thermal stability of V. cholerae L-asparaginase was evaluated. Compared to other thermal stabilizers tested, glycine was found to be more effective which resulted in five folds increase in halflife time of enzyme at 50 °C.
Estari Mamidala
Kakatiya University, India
Title: Potent anti-human immunodeficiency virus activity of 4',7-dihydroxy-3-methoxy- 5,6-dimethylflavone, a novel compound blocking HIV-1 reverse transcriptase
Biography:
Abstract:
Introduction: HIV is a major global concern due to its drug resistance and warrants the development of novel antivirals as an alternative therapy. This study aims to test the anti-HIV activity of a novel compound 4',7-dihydroxy-3-methoxy-5,6-dimethylflavone, isolated from Hydrastis canadensis.
Methods: The crude extracts were prepared from dried areal parts Hydrastis canadensis in methanol by Soxhlet method and isolated 4',7-dihydroxy-3-methoxy-5,6-dimethylflavone by using column chromatography and HPLC. Peripheral Blood Mononuclear Cells (PBMCs) isolated from healthy donors by Ficoll-Hypaque density gradient centrifugation method. Cell viability test was performed on 4',7-dihydroxy-3-methoxy-5,6- dimethylflavone by MTT assay using PBMC and HIV-1 RT inhibition activity was determined by HIV-1 Reverse Transcriptase (p66) Capture ELISA.
Results: In the HIV-1 reverse transcriptase assay, isolated compound showed good inhibitory activity (92%) at 110 μg/ml and MTT assay confirmed that the isolated 4',7-dihydroxy-3-methoxy-5,6-dimethylflavone had no cytotoxic activity and IC50 values higher than 100 μg/ml.
Conclusion: The compound isolated from Hydrastis canadensis seemed to have the potential to act as a source of useful drugs and also to improve the health status of the consumers as a result of the presence of active compound that are vital for good health.
Madhavi Maddala
Osmania University, India
Title: Elucidation of gene expression pattern of Solasodine treated Tribolium confusum in different growth developmental stages
Biography:
Madhavi Maddala has completed her PhD from Osmania University, India. She has published more than 25 papers in reputed journals and has been awarded as Fellow of Academy of Environmental Biology, India (FAEB), Associate Fellow of Telangana Academy of Sciences (AFTAS) and AP Academy of Sciences (APAS).
Abstract:
During the last two decades phytochemicals from plant bioresources have been heralded as desirable alternative to synthetic chemical insecticides for pest management because they pose little threat to the environment or human health. In our earlier studies, we reported the effect of commercial glycoalkaloid, Solasodine extracted from the green fruits of S. aviculare (Solanaceae) on T. confusum larvae. The treatment prevented the normal development of the last instar larvae. However, the molecular mechanism of the phytochemical effect on the larvae is yet to be studied. Lindsey C. Perkin corresponding and Brenda Oppert in recently reported the list of genes that are altered during developmental stages.
Madhavi Maddala
Osmania University, India
Title: Elucidation of gene expression pattern of Solasodine treated Tribolium confusum in different growth developmental stages
Biography:
Madhavi Maddala has completed her PhD from Osmania University, India. She has published more than 25 papers in reputed journals and has been awarded as Fellow of Academy of Environmental Biology, India (FAEB), Associate Fellow of Telangana Academy of Sciences (AFTAS) and AP Academy of Sciences (APAS).
Abstract:
During the last two decades phytochemicals from plant bioresources have been heralded as desirable alternative to synthetic chemical insecticides for pest management because they pose little threat to the environment or human health. In our earlier studies, we reported the effect of commercial glycoalkaloid, Solasodine extracted from the green fruits of S. aviculare (Solanaceae) on T. confusum larvae. The treatment prevented the normal development of the last instar larvae. However, the molecular mechanism of the phytochemical effect on the larvae is yet to be studied. Lindsey C. Perkin corresponding and Brenda Oppert in recently reported the list of genes that are altered during developmental stages.